Mouse Assay:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
Recombinant:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
Protease Inhibitor:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
cDNA Synthesis:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
SYBR Green Assay:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
Bicinchoninic Acid Protein Assay:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
Western Blot:Article Title: Steroidogenic Metabolism of Galeterone Reveals a Diversity of Biochemical Activities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies b-Actin Sigma-Aldrich CAT#A2228 AR Santa Cruz CAT#sc-815 Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31430 Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP Thermo Fisher Scientific CAT#31460 Biological Samples Double charcoal-stripped human serum Golden West Biological Inc CAT#SP2050 Mice Serum This paper N/A Chemicals, Peptides, and Recombinant Proteins b-glucuronidase Sigma-Aldrich CAT#G7646-500 Dutasteride Medkoo Biosciences CAS#164656-23-9 j Galeterone Shanghai Forever Synthesis Co., Ltd NX41765 D4G Shanghai Forever Synthesis Co., Ltd NX41765 Acetonitrile Thermo Fisher Scientific CAT#A9554 Methanol Thermo Fisher Scientific CAT#A454K4 Formic Acid Thermo Fisher Scientific CAT#A1170 poly-DL-ornithine Sigma-Aldrich CAT#P3655-1G [3H]-DHEA PerkinElmer NET 814001MC [3H]-AD PerkinElmer NET 926005MC [3H]-Pregnenolone PerkinElmer NET 039001MC [3H]-R1881 PerkinElmer NET 590250VC NaCl Thermo Fisher Scientific CAT#S 271-1 RIPA buffer Sigma-Aldrich CAT#R0278 Protease inhibitor cocktail tablet Roche 05892791001 DHEA pellets Innovative research of America CAT#A-111 Ethyl alcohol Thermo Fisher Scientific CAT#AC 615095000 Safflower oil Sigma-Aldrich CAT#47120-U Matrigel Thermo Fisher Scientific CB40234 Ethyl acetate Sigma-Aldrich CAT#34858-4L Iso octane Thermo Fisher Scientific CAT#03014 Tert-butyl methyl ether Thermo Fisher Scientific CAT#AC 389050025 Liquiscint scintillation cocktail Thermo Fisher Scientific CAT#5089990170 See Chemical Synthesis for synthesis for additional compound This paper Supplementary methods Critical Commercial Assays GenElute Mammalian Total RNA miniprep kit Sigma-Aldrich KIT#RTN 350 iScript cDNA Synthesis Kit Bio-Rad KIT#1708891 iTaq Fast SYBR Green Supermix with ROX Bio-Rad CAT#1725125 LipoD SignaGen Laboratories CAT#SL100668 Pierce BCA Protein Assay Kit Thermal Fisher Scientific CAT#23225 Pierce ECL Western Blotting Substrate Thermal Fisher Scientific CAT#32209 (Continued on next page) Cell Chemical Biology 24, 825–832.e1–e6, July 20, 2017 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Experimental Models: Cell Lines LNCaP ATCC CRL -1740 VCaP ATCC CRL -2876
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